Core Antibodies for tCyCIF (Image available, November 2018, Lin, et al. eLife 2018)

This is a work in progress. Our goal is to share annotated raw images for each antibodies tested.

You can navigate through, search, and sort (by clicking the table column heading) the following table.

This work is made available under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) License

Antibodies have been tested for a mimum level of perfomance as descrbed in footnotes below; this should not be assumed to suggest “validation” of the antibody

Search Antibodies
Antibody Name Target Protein Performance Image Vendor Catalog no. Clone Fluorophore Research Resource Identifier FILTER SET Note Link
CD4-488 CD4 *** View R&D Systems FAB8165G Polyclonal Alexa Fluor 488 488/FITC link
CD8a-488 CD8 *** View eBioscience 53-0008-80 AMC908 Alexa Fluor 488 AB_2574412 488/FITC link
cJUN-488 cJUN *** View Abcam AB193780 E254 Alexa Fluor 488 488/FITC link
Ecad-488 E-Cadherin *** View CST 3199 2.40E+11 Alexa Fluor 488 AB_10691457 488/FITC link
EGFR-488 EGFR *** View CST 5616 D38B1 Alexa Fluor 488 AB_10691853 488/FITC link
Ki67-488 Ki67 *** View CST 11882 D3B5 Alexa Fluor 488 AB_2687824 488/FITC link
LaminA/C-488 Lamin A/C *** View CST 8617 4C11 Alexa Fluor 488 AB_10997529 488/FITC link
MITF-488 MITF *** View Abcam AB201675 D5 Alexa Fluor 488 488/FITC link
PCNA-488 PCNA *** View CST 8580 PC10 Alexa Fluor 488 AB_11178664 488/FITC link
pERK-488 pERK(T202/Y204) *** View CST 4344 D13.14.4E Alexa Fluor 488 AB_10695876 488/FITC Discontinued link
S100a-488 S100alpha *** View Abcam AB207367 EPR5251 Alexa Fluor 488 488/FITC link
CD3D-555 CD3D *** View Abcam AB208514 EP4426 Alexa Fluor 555 555/Cy3 link
CD45-PE CD45 *** View R&D Systems FAB1430P-100 2D1 PE AB_2237898 555/Cy3 link
cMYC-555 cMYC *** View Abcam AB201780 Y69 Alexa Fluor 555 555/Cy3 link
E2F1-555 E2F1 *** View Abcam AB208078 EPR3818(3) Alexa Fluor 555 555/Cy3 link
FOXP3-570 FOXP3 *** View eBioscience 41-4777-80 236A/E7 eFluor 570 AB_2573608 555/Cy3 link
Keratin-570 pan-Keratin *** View eBioscience 41-9003-80 AE1/AE3 eFluor 570 AB_11217482 555/Cy3 link
Ki67-570 Ki67 *** View eBioscience 41-5699-80 20Raj1 eFluor 570 AB_11220088 555/Cy3 link
PDL1-555 PD-L1/CD274 *** View Abcam AB213358 28-8 Alexa Fluor 555 555/Cy3 link
pH3-555 pH3(S10) *** View CST 3475 D2C8 Alexa Fluor 555 AB_10694639 555/Cy3 link
VEGFR2-PE VEGFR2 *** View CST 12634 D5B1 PE 555/Cy3 link
Vimentin-555 Vimentin *** View CST 9855 D21H3 Alexa Fluor 555 AB_10859896 555/Cy3 link
aSMA-660 aSMA *** View eBioscience 50-9760-80 1A4 eFluor 660 AB_2574361 647/Cy5 link
Catenin-647 Beta-Catenin *** View CST 4627 L54E2 Alexa Fluor 647 AB_10691326 647/Cy5 link
CD8a-660 CD8 *** View eBioscience 50-0008-80 AMC908 eFluor 660 AB_2574148 647/Cy5 link
gH2ax-647 H2ax(S139) *** View BioLegend 613407 2F3 Alexa Fluor 647 AB_2114994 647/Cy5 link
HES1-647 HES1 *** View Abcam AB196577 EPR4226 Alexa Fluor 647 647/Cy5 link
MHCI-647 MHCI/HLAA *** View Abcam AB199837 EP1395Y Alexa Fluor 647 647/Cy5 link
NFkB-647 NFkB (p65) *** View Abcam AB190589 E379 Alexa Fluor 647 647/Cy5 link
NGFR-647 NGFR/CD271 *** View Abcam AB195180 EP1039Y Alexa Fluor 647 647/Cy5 link
PDL1-647 PD-L1/CD274 *** View CST 15005 E1L3N Alexa Fluor 647 647/Cy5 link
pSTAT3-647 pSTAT3(Y705) *** View CST 4324 D3A7 Alexa Fluor 647 AB_10694637 647/Cy5 link
Tubulin-647 beta-Tubulin *** View CST 3624 9F3 Alexa Fluor 647 AB_10694204 647/Cy5 link
Vimentin-647 Vimentin *** View BioLegend 677807 O91D3 Alexa Fluor 647 AB_2616801 647/Cy5 link
anti-pERK pERK(T202/Y204) *** View CST 4370 D13.14.4E N/D AB_2315112 N/D link
anti-pRB pRB(S807/811) *** View Santa Cruz SC-16670 Polyclonal N/D AB_655250 N/D Discontinued link
CD11b-555 CD11b ** View Abcam AB206616 EPR1344 Alexa Fluor 555 555/Cy3 link
PD1-647 PD1 ** View Abcam AB201825 EPR4877 (2) Alexa Fluor 647 647/Cy5 link
CD45RO-488 CD45RO * View BioLegend 304212 UCHL1 Alexa Fluor 488 AB_528823 488/FITC link
CD3-660 CD3 * View eBioscience 50-0037-41 OKT3 eFluor 660 AB_2574150 647/Cy5 link

*** Show positive/correct signals in mutliple samples/tissues as judged by eye

** Show positive/correct signals in some but not all samples tested as judged by eye

* show no signal or obviously incorrect signals in all samples tested