Antibodies Tested for Tissue-based CyCIF (Full table, May 2018, Lin, et al. eLife 2018)

You can navigate through, search, and sort (by clicking the table column heading) the following table as well as download a spreadsheet copy.

This work is made available under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) License

Antibodies have been tested for a mimum level of perfomance as descrbed in footnotes below; this should not be assumed to suggest “validation” of the antibody

Search Antibodies
Antibody Name Target Protein Performance Vendor Catalog no. Clone Fluorophore Research Resource Identifier FILTER SET Note Link
Bax-488 Bax *** BioLegend 633603 2D2 Alexa Fluor 488 AB_2562171 488/FITC link
CD11b-488 CD11b *** Abcam AB204271 EPR1344 Alexa Fluor 488 488/FITC link
CD4-488 CD4 *** R&D Systems FAB8165G Polyclonal Alexa Fluor 488 488/FITC link
CD8a-488 CD8 *** eBioscience 53-0008-80 AMC908 Alexa Fluor 488 AB_2574412 488/FITC link
cJUN-488 cJUN *** Abcam AB193780 E254 Alexa Fluor 488 488/FITC link
CK18-488 Cytokeratin 18 *** eBioscience 53-9815-80 LDK18 Alexa Fluor 488 AB_2574480 488/FITC link
CK8-FITC Cytokeratin 8 *** eBioscience 11-9938-80 LP3K FITC AB_10548518 488/FITC link
CycD1-488 CycD1 *** Abcam AB190194 EPR2241 Alexa Fluor 488 488/FITC link
Ecad-488 E-Cadherin *** CST 3199 24E10 Alexa Fluor 488 AB_10691457 488/FITC link
EGFR-488 EGFR *** CST 5616 D38B1 Alexa Fluor 488 AB_10691853 488/FITC link
EpCAM-488 EpCAM *** CST 5198 VU1D9 Alexa Fluor 488 AB_10692105 488/FITC link
HES1-488 HES1 *** Abcam AB196328 EPR4226 Alexa Fluor 488 488/FITC link
Ki67-488 Ki67 *** CST 11882 D3B5 Alexa Fluor 488 AB_2687824 488/FITC link
LaminA/C-488 Lamin A/C *** CST 8617 4C11 Alexa Fluor 488 AB_10997529 488/FITC link
LaminB1-488 Lamin B1 *** Abcam AB194106 EPR8985(B) Alexa Fluor 488 488/FITC link
mCD3E-FITC ms_CD3E *** BioLegend 100306 145-2C11 FITC AB_312671 488/FITC link
mCD4-488 ms_CD4 *** BioLegend 100532 RM4-5 Alexa Fluor 488 AB_493373 488/FITC link
MET-488 c-MET *** CST 8494 D1C2 Alexa Fluor 488 AB_10999405 488/FITC link
mF4/80-488 ms_F4/80 *** BioLegend 123120 BM8 Alexa Fluor 488 AB_893479 488/FITC link
MITF-488 MITF *** Abcam AB201675 D5 Alexa Fluor 488 488/FITC link
Ncad-488 N-Cadherin *** BioLegend 350809 8C11 Alexa Fluor 488 AB_11218797 488/FITC link
p53-488 p53 *** CST 5429 7F5 Alexa Fluor 488 AB_10695458 488/FITC link
PCNA-488 PCNA *** CST 8580 PC10 Alexa Fluor 488 AB_11178664 488/FITC link
PD1-488 PD1 *** CST 15131 D3W4U Alexa Fluor 488 488/FITC Discontinued link
PDI-488 PDI *** CST 5051 C81H6 Alexa Fluor 488 AB_10950503 488/FITC link
pERK-488 pERK(T202/Y204) *** CST 4344 D13.14.4E Alexa Fluor 488 AB_10695876 488/FITC Discontinued link
pNDG1-488 pNDG1(T346) *** CST 6992 D98G11 Alexa Fluor 488 AB_10827648 488/FITC link
POL2A-488 POL2A *** Novus Biologicals NB200-598AF488 4H8 Alexa Fluor 488 AB_2167465 488/FITC link
pS6(S240/244)-488 pS6(240/244) *** CST 5018 D68F8 Alexa Fluor 488 AB_10695861 488/FITC link
S100a-488 S100alpha *** Abcam AB207367 EPR5251 Alexa Fluor 488 488/FITC link
SQSTM1-488 SQSTM1/p62 *** CST 8833 D1D9E3 Alexa Fluor 488 488/FITC Discontinued link
STAT3-488 STAT3 *** CST 14047 B3Z2G Alexa Fluor 488 488/FITC link
Survivin-488 Survivin *** CST 2810 71G4B7 Alexa Fluor 488 AB_10691462 488/FITC link
Catenin-488 β-Catenin *** CST 2849 L54E2 Alexa Fluor 488 AB_10693296 488/FITC link
Actin-555 Actin *** CST 8046 13E5 Alexa Fluor 555 AB_11179208 555/Cy3 link
CD11c-570 CD11c *** eBioscience 41-9761-80 118/A5 eFluor 570 AB_2573632 555/Cy3 link
CD3D-555 CD3D *** Abcam AB208514 EP4426 Alexa Fluor 555 555/Cy3 link
CD4-570 CD4 *** eBioscience 41-2444-80 N1UG0 eFluor 570 AB_2573601 555/Cy3 link
CD45-PE CD45 *** R&D Systems FAB1430P-100 2D1 PE AB_2237898 555/Cy3 link
CK7-555 Cytokeratin 7 *** Abcam AB209601 EPR17078 Alexa Fluor 555 555/Cy3 link
cMYC-555 cMYC *** Abcam AB201780 Y69 Alexa Fluor 555 555/Cy3 link
E2F1-555 E2F1 *** Abcam AB208078 EPR3818(3) Alexa Fluor 555 555/Cy3 link
Ecad-555 E-Cadherin *** CST 4295 24E10 Alexa Fluor 555 555/Cy3 Discontinued link
EpCAM-PE EpCAM *** BioLegend 324205 9C4 PE AB_756079 555/Cy3 link
FOXO1a-555 FOXO1a *** Abcam AB207244 EP927Y Alexa Fluor 555 555/Cy3 link
FOXP3-570 FOXP3 *** eBioscience 41-4777-80 236A/E7 eFluor 570 AB_2573608 555/Cy3 link
GFAP-570 GFAP *** eBioscience 41-9892-80 GA5 eFluor 570 AB_2573655 555/Cy3 link
HSP90-PE HSP90b *** Abcam AB115641 Polyclonal PE AB_10936222 555/Cy3 link
KAP1-594 KAP1 *** BioLegend 619304 20A1 Alexa Fluor 594 AB_2563298 555/Cy3 Hard to inactivate link
Keratin-555 pan-Keratin *** CST 3478 C11 Alexa Fluor 555 AB_10829040 555/Cy3 link
Keratin-570 pan-Keratin *** eBioscience 41-9003-80 AE1/AE3 eFluor 570 AB_11217482 555/Cy3 link
Ki67-570 Ki67 *** eBioscience 41-5699-80 20Raj1 eFluor 570 AB_11220088 555/Cy3 link
LC3-555 LC3 *** CST 13173 D3U4C Alexa Fluor 555 555/Cy3 link
MAP2-570 MAP2 *** eBioscience 41-9763-80 AP20 eFluor 570 AB_2573634 555/Cy3 link
pAUR-555 pAUR1/2/3(T288/T232/T198) *** CST 13464 D13A11 Alexa Fluor 555 555/Cy3 link
pCHK2-PE pChk2(T68) *** CST 12812 C13C1 PE 555/Cy3 link
PDL1-555 PD-L1/CD274 *** Abcam AB213358 28-8 Alexa Fluor 555 555/Cy3 link
pH3-555 pH3(S10) *** CST 3475 D2C8 Alexa Fluor 555 AB_10694639 555/Cy3 link
pRB-555 pRB(S807/811) *** CST 8957 D20B12 Alexa Fluor 555 555/Cy3 link
pS6(235/236)-555 pS6(235/236) *** CST 3985 D57.2.2E Alexa Fluor 555 AB_10693792 555/Cy3 Discontinued link
pSRC-PE pSRC(Y418) *** eBioscience 12-9034-41 SC1T2M3 PE AB_2572680 555/Cy3 link
S6-555 S6 *** CST 6989 54D2 Alexa Fluor 555 AB_10828226 555/Cy3 link
SQSTM1-555 SQSTM1/p62 *** Abcam AB203430 EPR4844 Alexa Fluor 555 555/Cy3 link
VEGFR2-555 VEGFR2 *** CST 12872 D5B1 Alexa Fluor 555 555/Cy3 Discontinued link
VEGFR2-PE VEGFR2 *** CST 12634 D5B1 PE 555/Cy3 link
Vimentin-555 Vimentin *** CST 9855 D21H3 Alexa Fluor 555 AB_10859896 555/Cy3 link
Vinculin-570 Vinculin *** eBioscience 41-9777-80 7F9 eFluor 570 AB_2573646 555/Cy3 link
gH2ax-PE gH2ax *** BioLegend 613412 2F3 PE AB_2616871 555/Cy5 link
AKT-647 AKT *** CST 5186 C67E7 Alexa Fluor 647 AB_10695877 647/Cy5 link
aSMA-660 aSMA *** eBioscience 50-9760-80 1A4 eFluor 660 AB_2574361 647/Cy5 link
B220-647 CD45R/B220 *** BioLegend 103226 RA3-6B2 Alexa Fluor 647 AB_389330 647/Cy5 link
Bcl2-647 Bcl2 *** BioLegend 658705 100 Alexa Fluor 647 AB_2563279 647/Cy5 link
Catenin-647 Beta-Catenin *** CST 4627 L54E2 Alexa Fluor 647 AB_10691326 647/Cy5 link
CD20-660 CD20 *** eBioscience 50-0202-80 L26 eFluor 660 AB_11151691 647/Cy5 link
CD45-647 CD45 *** BioLegend 304020 HI30 Alexa Fluor 647 AB_493034 647/Cy5 link
CD8a-660 CD8 *** eBioscience 50-0008-80 AMC908 eFluor 660 AB_2574148 647/Cy5 link
CK5-647 Cytokeratin 5 *** Abcam AB193895 EP1601Y Alexa Fluor 647 647/Cy5 link
CoIIV-647 Collagen IV *** eBioscience 51-9871-80 1042 Alexa Fluor 647 AB_10854267 647/Cy5 link
COXIV-647 COXIV *** CST 7561 3E11 Alexa Fluor 647 AB_10994876 647/Cy5 link
cPARP-647 cPARP *** CST 6987 D64E10 Alexa Fluor 647 AB_10858215 647/Cy5 link
FOXA2-660 FOXA2 *** eBioscience 50-4778-82 3C10 eFluor 660 AB_2574221 647/Cy5 link
FOXP3-647 FOXP3 *** BioLegend 320113 206D Alexa Fluor 647 AB_439753 647/Cy5 link
gH2ax-647 H2ax(S139) *** CST 9720 20E3 Alexa Fluor 647 AB_10692910 647/Cy5 link
gH2ax-647 H2ax(S139) *** BioLegend 613407 2F3 Alexa Fluor 647 AB_2114994 647/Cy5 link
HES1-647 HES1 *** Abcam AB196577 EPR4226 Alexa Fluor 647 647/Cy5 link
Ki67-647 Ki67 *** CST 12075 D3B5 Alexa Fluor 647 647/Cy5 link
Ki67-647 Ki67 *** BioLegend 350509 Ki-67 Alexa Fluor 647 AB_10900810 647/Cy5 link
mCD45-647 ms_CD45 *** BioLegend 103124 30-F11 Alexa Fluor 647 AB_493533 647/Cy5 link
mCD4-647 ms_CD4 *** BioLegend 100426 GK1.5 Alexa Fluor 647 AB_493519 647/Cy5 link
mEPCAM-647 ms_EPCAM *** BioLegend 118211 G8.8 Alexa Fluor 647 AB_1134104 647/Cy5 link
MHCI-647 MHCI/HLAA *** Abcam AB199837 EP1395Y Alexa Fluor 647 647/Cy5 link
MHCII-647 MHCII *** Abcam AB201347 EPR11226 Alexa Fluor 647 647/Cy5 link
mLy6C-647 ms_Ly6C *** BioLegend 128009 HK1.4 Alexa Fluor 647 AB_1236551 647/Cy5 link
mTOR-647 mTOR *** CST 5048 7C10 Alexa Fluor 647 AB_10828101 647/Cy5 link
NFkB-647 NFkB (p65) *** Abcam AB190589 E379 Alexa Fluor 647 647/Cy5 link
NGFR-647 NGFR/CD271 *** Abcam AB195180 EP1039Y Alexa Fluor 647 647/Cy5 link
NUP98-647 NUP98 *** CST 13393 C39A3 Alexa Fluor 647 647/Cy5 link
p21-647 p21 *** CST 8587 12D1 Alexa Fluor 647 AB_10892861 647/Cy5 link
p27-647 p27 *** Abcam AB194234 Y236 Alexa Fluor 647 647/Cy5 link
pATM-660 pATM(S1981) *** eBioscience 50-9046-41 10H11.E12 eFluor 660 AB_2574312 647/Cy5 link
PAX8-647 PAX8 *** Abcam AB215953 EPR18715 Alexa Fluor 647 647/Cy5 link
PDL1-647 PD-L1/CD274 *** CST 15005 E1L3N Alexa Fluor 647 647/Cy5 link
pMK2-647 pMK2(T334) *** CST 4320 27B7 Alexa Fluor 647 AB_10695401 647/Cy5 Discontinued link
pmTOR-660 pmTOR(S2448) *** eBioscience 50-9718-41 MRRBY eFluor 660 AB_2574351 647/Cy5 link
pS6_235-647 pS6(S235/S236) *** CST 4851 D57.2.2E Alexa Fluor 647 AB_10695457 647/Cy5 link
pSTAT3-647 pSTAT3(Y705) *** CST 4324 D3A7 Alexa Fluor 647 AB_10694637 647/Cy5 link
pTyr-647 p-Tyrosine *** CST 9415 p-Tyr-100 Alexa Fluor 647 AB_10693160 647/Cy5 link
S100A4-647 S100A4 *** Abcam AB196168 EPR2761(2) Alexa Fluor 647 647/Cy5 link
Survivin-647 Survivin *** CST 2866 71G4B7 Alexa Fluor 647 AB_10698609 647/Cy5 link
TUBB3-647 TUBB3 *** BioLegend 657405 AA10 Alexa Fluor 647 AB_2563609 647/Cy5 link
Tubulin-647 beta-Tubulin *** CST 3624 9F3 Alexa Fluor 647 AB_10694204 647/Cy5 link
Vimentin-647 Vimentin *** BioLegend 677807 O91D3 Alexa Fluor 647 AB_2616801 647/Cy5 link
anti-14-3-3 14-3-3 *** Santa Cruz SC-629-G Polyclonal N/D AB_630820 N/D link
anti-53BP1 53BP1 *** Bethyl A303-906A Polyclonal N/D AB_2620256 N/D link
anti-5HMC 5HMC *** Active Motif 39769 Polyclonal N/D AB_10013602 N/D link
anti-CD11b CD11b *** Abcam AB133357 EPR1344 N/D AB_2650514 N/D link
anti-CD2 CD2 *** Abcam AB37212 Polyclonal N/D AB_726228 N/D link
anti-CD20 CD20 *** Dako M0755 L26 N/D AB_2282030 N/D link
anti-CD3 CD3 *** Dako A0452 Polyclonal N/D AB_2335677 N/D link
anti-CD4 CD4 *** Dako M7310 4B12 N/D N/D link
anti-CD45RO CD45RO *** Dako M0742 UCHL1 N/D AB_2237910 N/D link
anti-CD8 CD8 *** Dako M7103 C8/144B N/D AB_2075537 N/D link
anti-CycA2 CycA2 *** Abcam AB38 E23.1 N/D AB_304084 N/D link
anti-ET1 ET-1 *** Abcam AB2786 TR.ET.48.5 N/D AB_303299 N/D link
anti-FAP FAP *** eBioscience BMS168 F11-24 N/D AB_10597443 N/D link
anti-FOXP3 FOXP3 *** BioLegend 320102 206D N/D AB_430881 N/D link
anti-LAMP2 LAMP2 *** Abcam AB25631 H4B4 N/D AB_470709 N/D link
anti-MCM6 MCM6 *** Santa Cruz SC-9843 Polyclonal N/D AB_2142543 N/D link
anti-PAX8 PAX8 *** Abcam AB191870 EPR18715 N/D N/D link
anti-PD1 PD1 *** CST 86163 D4W2J N/D N/D link
anti-pEGFR pEGFR(Y1068) *** CST 3777 D7A5 N/D AB_2096270 N/D link
anti-pERK pERK(T202/Y204) *** CST 4370 D13.14.4E N/D AB_2315112 N/D link
anti-pRB pRB(S807/811) *** Santa Cruz SC-16670 Polyclonal N/D AB_655250 N/D Discontinued link
anti-pRPA32 pRPA32 (S4/S8) *** Bethyl IHC-00422 Polyclonal N/D AB_1659840 N/D link
anti-pSTAT3 pSTAT3 *** CST 9145 D3A7 N/D AB_2491009 N/D link
anti-pTyr pTyr *** CST 9411 p-Tyr-100 N/D AB_331228 N/D link
anti-RPA32 RPA32 *** Bethyl IHC-00417 Polyclonal N/D AB_1659838 N/D link
anti-TPCN2 TPCN2 *** NOVUSBIO NBP1-86923 Polyclonal N/D AB_11021735 N/D link
anti-VEGFR1 VEGFR1/FLT1 *** Santa Cruz SC-31173 Polyclonal N/D AB_2106885 N/D link
Abeta-488 Beta-Amyloid (1-16) ** BioLegend 803013 6E10 Alexa Fluor 488 AB_2564765 488/FITC link
BRAF-FITC B-RAF ** Abcam ab175637 K21-F FITC 488/FITC link
BrdU-488 BrdU ** BioLegend 364105 3D4 Alexa Fluor 488 AB_2564499 488/FITC link
cCasp3-488 cCasp3 ** R&D Systems IC835G-025 269518 Alexa Fluor 488 488/FITC link
CD11b-488 CD11b ** BioLegend 101219 M1/70 Alexa Fluor 488 AB_493545 488/FITC link
CD123-488 CD123 ** BioLegend 306035 6H6 Alexa Fluor 488 AB_2629569 488/FITC link
CD49b-FITC CD49b ** BioLegend 359305 P1E6-C5 FITC AB_2562530 488/FITC link
CD69-FITC CD69 ** BioLegend 310904 FN50 FITC AB_314839 488/FITC link
CD71-FITC CD71 ** BioLegend 334103 CY1G4 FITC AB_1236432 488/FITC link
CD80-FITC CD80 ** R&D Systems FAB140F 37711 FITC AB_357027 488/FITC link
CD8a-488 CD8a ** eBioscience 53-0086-41 OKT8 Alexa Fluor 488 AB_10547060 488/FITC link
CDC2-FITC CDC2/p34 ** Santa Cruz SC-54 FITC 17 FITC AB_627224 488/FITC link
CycB1-FITC CycB1 ** Santa Cruz SC-752 FITC Polyclonal FITC AB_2072134 488/FITC link
FN-488 Fibronection ** Abcam AB198933 F1 Alexa Fluor 488 488/FITC link
IFNG-488 Interferron-Gamma ** BioLegend 502517 4S.B3 Alexa Fluor 488 AB_493030 488/FITC link
IL1-FITC IL1 ** BioLegend 511705 H1b-98 FITC AB_1236434 488/FITC link
IL6-FITC IL6 ** BioLegend 501103 MQ2-13A5 FITC AB_315151 488/FITC link
mCD31-FITC ms_CD31 ** eBioscience 11-0311-82 390 FITC AB_465012 488/FITC link
mCD8a-488 ms_CD8a ** BioLegend 100726 53-6.7 Alexa Fluor 488 AB_493423 488/FITC link
Nestin-488 Nestin ** eBioscience 53-9843-80 10C2 Alexa Fluor 488 AB_1834347 488/FITC link
NeuN-488 NeuN ** Millipore MAB377X A60 Alexa Fluor 488 AB_2149209 488/FITC link
PR-488 PR/PGR ** Abcam AB199224 YR85 Alexa Fluor 488 488/FITC link
Snail1-488 Snail1 ** eBioscience 53-9859-80 20C8 Alexa Fluor 488 AB_2574482 488/FITC link
TGFB-FITC TGFB1 ** BioLegend 349605 TW4-2F8 FITC AB_10679043 488/FITC link
TNFa-488 TNFa ** BioLegend 502917 MAb11 Alexa Fluor 488 AB_493122 488/FITC link
AR-555 AR ** CST 8956 D6F11 Alexa Fluor 555 AB_11129223 555/Cy3 link
CD11a-PE CD11a ** BioLegend 301207 HI111 PE AB_314145 555/Cy3 link
CD11b-555 CD11b ** Abcam AB206616 EPR1344 Alexa Fluor 555 555/Cy3 link
CD131-PE CD131 ** BD 559920 JORO50 PE AB_397374 555/Cy3 link
CD14-PE CD14 ** eBioscience 12-0149 61D3 PE AB_10597598 555/Cy3 link
CD1a-PE CD1a ** BioLegend 300105 HI149 PE AB_314019 555/Cy3 link
CD1c-PE CD1c ** BioLegend 331505 L161 PE AB_1089000 555/Cy3 link
CD20-PE CD20 ** BioLegend 302305 2H7 PE AB_314253 555/Cy3 link
CD23-PE CD23 ** eBioscience 12-0232-81 B3B4 PE AB_465592 555/Cy3 link
CD31-PE CD31 ** eBioscience 12-0319-41 WM-59 PE AB_10670623 555/Cy3 link
CD31-PE CD31 ** R&D Systems FAB3567P-025 9G11 PE AB_2279388 555/Cy3 link
CD34-PE CD34 ** Abcam AB30377 QBEND/10 PE AB_726407 555/Cy3 link
CD45R-e570 CD45R/B220 ** eBioscience 41-0452-80 RA3-6B2 eFluor 570 AB_2573598 555/Cy3 link
CD71-PE CD71 ** eBioscience 12-0711-81 R17217 PE AB_465739 555/Cy3 link
CD86-PE CD86 ** BioLegend 305405 IT2.2 PE AB_314525 555/Cy3 link
CK19-570 Cytokeratin 19 ** eBioscience 41-9898-80 BA17 eFluor 570 AB_11218678 555/Cy3 link
HER2-570 HER2 ** eBioscience 41-9757-80 MJD2 eFluor 570 AB_2573628 555/Cy3 link
IL3-PE IL3 ** BD 554383 MP2-8F8 PE AB_395358 555/Cy3 link
NFATc1-PE NFATc1 ** BioLegend 649605 7A6 PE AB_2562546 555/Cy3 link
PDL1-PE PD-L1/CD274 ** BioLegend 329705 29E.2A3 PE AB_940366 555/Cy3 link
pMAPK (T202/Y204) pERK1/2(T202/Y204) ** CST 14095 197G2 PE 555/Cy3 link
pMAPK (Y204/Y187) pERK1/2(Y204/Y187) ** CST 75165 D1H6G PE 555/Cy3 link
pSTAT1-PE pSTAT1(Y705) ** BioLegend 686403 A15158B PE AB_2616938 555/Cy3 link
ABCC1-647 ABCC1 ** BioLegend 370203 QCRL-2 Alexa Fluor 647 AB_2566664 647/Cy5 link
AnnexinV-674 N/D ** BioLegend 640911 NA Alexa Fluor 647 AB_2561293 647/Cy5 link
CD103-647 CD103 ** BioLegend 350209 Ber-ACT8 Alexa Fluor 647 AB_10640870 647/Cy5 link
CD25-647 CD25 ** BioLegend 302617 BC96 Alexa Fluor 647 AB_493046 647/Cy5 link
CD31-APC CD31 ** eBioscience 17-0319-41 WM-59 APC AB_10853188 647/Cy5 link
CD68-APC CD68 ** BioLegend 333809 Y1/82A APC AB_10567107 647/Cy5 link
CD8a-647 CD8a ** BioLegend 344725 SK1 Alexa Fluor 647 AB_2563451 647/Cy5 link
CD8a-647 CD8a ** R&D Systems FAB1509R-025 37006 Alexa Fluor 647 647/Cy5 link
CycE-660 CycE ** eBioscience 50-9714-80 HE12 eFluor 660 AB_2574350 647/Cy5 link
HIF1-647 HIF1 ** BioLegend 359705 546-16 Alexa Fluor 647 AB_2563331 647/Cy5 link
HP1-647 HP1 ** Abcam AB198391 EPR5777 Alexa Fluor 647 647/Cy5 link
mCD123-APC ms_CD123 ** eBioscience 17-1231-81 5B11 APC AB_891363 647/Cy5 link
NGFR-647 NGFR/CD271 ** BD 560326 C40-1457 Alexa Fluor 647 AB_1645403 647/Cy5 link
pBTK-660 pBTK(Y551/Y511) ** eBioscience 50-9015-80 M4G3LN eFluor 660 AB_2574306 647/Cy5 link
PD1-647 PD1 ** Abcam AB201825 EPR4877 (2) Alexa Fluor 647 647/Cy5 link
PR-660 PR/PGR ** eBioscience 50-9764-80 KMC912 eFluor 660 AB_2574363 647/Cy5 link
RUNX3-660 RUNX3 ** eBioscience 50-9817-80 R3-5G4 eFluor 660 AB_2574383 647/Cy5 link
SOX2-647 SOX2 ** Abcam AB192075 Polyclonal Alexa Fluor 647 647/Cy5 link
anti-53BP1 53BP1 ** Millipore MAB3802  BP13 N/D AB_2206767 N/D link
anti-Axl Axl ** R&D AF154 Polyclonal N/D AB_354852 N/D link
anti-CD11b CD11b ** Abcam AB52478 EP1345Y N/D AB_868788 N/D link
anti-CD8a CD8 ** eBioscience 14-0085-80 C8/144B N/D AB_11151339 N/D link
anti-CEP170 CEP170 ** Abcam AB72505 Polyclonal N/D AB_1268101 N/D link
anti-cMYC cMYC ** BioLegend 626801 9E10 N/D AB_2235686 N/D link
anti-CPS1 CPS1 ** Abcam AB129076 EPR7493-3 N/D AB_11156290 N/D link
anti-E2F1 E2F1 ** ThermoFisher MS-879-P1 KH95 N/D AB_143934 N/D link
anti-eEF2K eEF2K ** Santa Cruz SC-21642 K-19 N/D AB_640043 N/D link
anti-Emil1 Emil1 ** Abcam AB212397 EMIL/1176 N/D N/D link
anti-FKHRL1 FKHRL1 ** Santa Cruz SC-9812 Polyclonal N/D AB_640608 N/D Discontinued link
anti-FLAG FLAG ** Sigma F1804 M2 N/D AB_262044 N/D link
anti-GranB Granzyme_B ** Dako M7235 M7235 N/D AB_2114697 N/D link
anti-HMB45 HMB45 ** Abcam AB732 HMB45+M2-7C10+M2-9E3 N/D AB_305844 N/D link
anti-HSP90b HSP90b ** Santa Cruz SC-1057 D-19 N/D AB_2121392 N/D link
anti-IL2Ra IL2Ra ** Abcam AB128955 EPR6452 N/D AB_11141054 N/D link
anti-LAMP2 LAMP2 ** R&D AF6228 Polyclonal N/D AB_10971818 N/D link
anti-MITF MITF ** Abcam AB12039 C5 N/D AB_298801 N/D link
anti-Ncad N-Cadherin ** Abcam AB18203 Polyclonal N/D AB_444317 N/D link
anti-NCAM NCAM ** Abcam AB6123 ERIC-1 N/D AB_2149537 N/D link
anti-NF1 NF1 ** Abcam AB178323 McNFn27b N/D N/D link
anti-pCTD Pol II CTD(S2) ** Active Motif 61083 3E10 N/D AB_2687450 N/D link
anti-PD1 PD1 ** CST 43248 EH33 N/D N/D link
anti-pTuberin pTuberin(S664) ** Abcam AB133465 EPR8202 N/D AB_11157389 N/D link
anti-S100 S100 ** Dako Z0311 Polyclonal N/D AB_10013383 N/D link
anti-SIRT3 SIRT3 ** CST 2627 C73E3 N/D AB_2188622 N/D link
anti-TIA1 TIA1 ** Santa Cruz SC-1751 Polyclonal N/D AB_2201433 N/D link
anti-TLR3 TLR3 ** Santa Cruz SC-8691 Polyclonal N/D AB_2240700 N/D link
anti-TNFa TNFa ** Abcam AB11564 MP6-XT3 N/D AB_298170 N/D link
anti-TPCN2 TPCN2 ** Abcam AB119915 Polyclonal N/D AB_10903692 N/D link
CD11a-FITC CD11a * eBioscience 11-0119-41 HI111 FITC AB_10597888 488/FITC link
CD20-FITC CD20 * BioLegend 302303 2H7 FITC AB_314251 488/FITC link
CD2-FITC CD2 * BioLegend 300206 RPA-2.10 FITC AB_314030 488/FITC link
CD45RO-488 CD45RO * BioLegend 304212 UCHL1 Alexa Fluor 488 AB_528823 488/FITC link
CD8a-488 CD8 * BioLegend 301024 RPA-T8 Alexa Fluor 488 AB_2561282 488/FITC link
cJUN-FITC cJUN * Santa Cruz SC-1694 FITC Polyclonal FITC AB_631263 488/FITC Discontinued link
CXCR5-FITC CXCR5 * BioLegend 356913 J252D4 FITC AB_2561895 488/FITC link
Ecad-FITC Ecad * BioLegend 324103 67A4 FITC AB_756065 488/FITC link
FOXP3-488 FOXP3 * BioLegend 320011 150D Alexa Fluor 488 AB_439747 488/FITC link
MITF-488 MITF * Novus Biologicals NB100-56561AF488 21D1418 Alexa Fluor 488 AB_838580 488/FITC link
NCAM-488 NCAM/CD56 * Abcam AB200333 EPR2566 Alexa Fluor 488 488/FITC link
NCAM-FITC NCAM/CD56 * ThermoFisher 11-0566-41 TULY56 FITC AB_2572458 488/FITC link
NGFR-FITC NGFR/CD271 * BioLegend 345103 ME20.4 FITC AB_1937226 488/FITC link
PD1-488 PD-1 * BioLegend 367407 NAT105 Alexa Fluor 488 AB_2566677 488/FITC link
PD1-488 PD-1 * BioLegend 329935 EH12.2H7 Alexa Fluor 488 AB_2563593 488/FITC link
pERK-488 pERK(T202/Y204) * CST 4374 E10 Alexa Fluor 488 AB_10705598 488/FITC link
pERK-488 pERK(T202/Y204) * CST 4780 137F5 Alexa Fluor 488 AB_10705598 488/FITC link
S100A4-FITC S100A4 * BioLegend 370007 NJ-4F3-D1 FITC AB_2572073 488/FITC link
SOX2-488 SOX2 * BioLegend 656109 14A6A34 Alexa Fluor 488 AB_2563956 488/FITC link
CD133-PE CD133 * eBioscience 12-1338-41 TMP4 PE AB_1582258 555/Cy3 link
cMyc-TRITC cMYC * Santa Cruz SC-40 TRITC 9E10 TRITC AB_627268 555/Cy3 link
cPARP-555 cPARP * CST 6894 D64E10 Alexa Fluor 555 AB_10830735 555/Cy3 link
CTLA4-PE CTLA4 * BioLegend 369603 BNI3 PE AB_2566796 555/Cy3 link
GATA3-594 GATA3 * BioLegend 653816 16E10A23 Alexa Fluor 594 AB_2563353 555/Cy3 link
GFAP-Cy3 GFAP * Millipore MAB3402C3 NA Cy3 AB_11213580 555/Cy3 link
Oct4-555 OCT_4 * CST 4439 C30A3 Alexa Fluor 555 AB_10922586 555/Cy3 link
p21-555 p21 * CST 8493 12D1 Alexa Fluor 555 AB_10860074 555/Cy3 link
PD1-PE PD1 * BioLegend 329905 EH12.2H7 PE AB_940481 555/Cy3 link
PDGFRb-555 PDGFRb * Abcam AB206874 Y92 Alexa Fluor 555 555/Cy3 link
pSTAT1-555 pSTAT1 * CST 8183 58D6 Alexa Fluor 555 AB_10860600 555/Cy3 link
TIM1-PE TIM1 * BioLegend 353903 1D12 PE AB_11125165 555/Cy3 link
cCasp3-647 cCasp3 * CST 9602 D3E9 Alexa Fluor 647 AB_2687881 647/Cy5 link
CD103-APC CD103 * eBioscience 17-1038-41 B-Ly7 APC AB_10669816 647/Cy5 link
CD3-647 CD3 * BioLegend 300422 UCHT1 Alexa Fluor 647 AB_493092 647/Cy5 link
CD3-660 CD3 * eBioscience 50-0037-41 OKT3 eFluor 660 AB_2574150 647/Cy5 link
CD3-APC CD3 * eBioscience 17-0038-41 UCHT1 APC AB_10804761 647/Cy5 link
CD45RO-APC CD45RO * BioLegend 304210 UCHL1 APC AB_314426 647/Cy5 link
ER-647 ER * Abcam AB205851 EPR4097 Alexa Fluor 647 647/Cy5 link
FOXO3a-647 FOXO3a * Abcam AB196539 EP1949Y Alexa Fluor 647 647/Cy5 link
GZMA-e660 granzyme A * ThermoFisher 50-9177-41 CB9 eFluor 660 AB_2574330 647/Cy5 link
GZMB-647 Granzyme_B * BioLegend 515405 GB11 Alexa Fluor 647 AB_2294995 647/Cy5 link
GZMB-APC Granzyme_B * R&D Systems IC29051A 356412 APC AB_894691 647/Cy5 Discontinued link
HER2-647 HER2 * BioLegend 324412 24D2 Alexa Fluor 647 AB_2262300 647/Cy5 link
mCD49b-647 ms_CD49b * BioLegend 103511 HMα2 Alexa Fluor 647 AB_528830 647/Cy5 link
NCAM-647 NCAM/CD56 * BioLegend 362513 5.1H11 Alexa Fluor 647 AB_2564086 647/Cy5 link
NCAM-e660 NCAM/CD56 * ThermoFisher 50-0565-80 5tukon56 eFluor 660 AB_2574160 647/Cy5 link
pAKT-647 pAKT * CST 4075 D9E Alexa Fluor 647 AB_10691856 647/Cy5 link
pERK-647 pERK (T202/Y204) * CST 4375 E10 Alexa Fluor 647 AB_10706777 647/Cy5 link
pERK-647 pERK (T202/Y204) * BioLegend 369503 6B8B69 Alexa Fluor 647 AB_2571895 647/Cy5 link
pIKBa-660 pIKBa * eBioscience 50-9035-41 RILYB3R eFluor 660 AB_2574310 647/Cy5 link
YAP-647 YAP * CST 38707S D8H1X Alexa Fluor 647 647/Cy5 link
anit-FANCD2 FANCD2 * Bethyl IHC-00624 Polyclonal N/D AB_10752755 N/D link
anit-pcJUN p-cJUN * Santa Cruz SC-822 KM-1 N/D AB_627262 N/D link
anti-AXL AXL * CST 8661 C89E7 N/D AB_11217435 N/D link
anti-CXCR5 CXCR5 * GeneTex GTX100351 Polyclonal N/D AB_1240668 N/D link
anti-CXCR5 CXCR5 * R&D MAB-190-SP 51505 N/D AB_2292654 N/D link
anti-FOXO3a FOXO3a * CST 2497 75D8 N/D AB_836876 N/D link
anti-GZMB Granzyme B * Abcam AB4059 Polyclonal N/D AB_304251 N/D link
anti-PD1 PD-1 * Abcam AB63477 Polyclonal N/D AB_2159165 N/D link
anti-PD1 PD-1 * ThermoFisher 14-9985-81 J43 N/D AB_468663 N/D link
anti-PD1 PD-1 * R&D AF1021 Polyclonal N/D AB_354541 N/D link
anti-RFP RFP * ThermoFisher R10367 Polyclonal N/D AB_2315269 N/D link
CD11C-BV570 CD11C * BioLegend 117331 N418 BV570 AB_10900261 N/D link
CD45-BV785 CD45 * BioLegend 304047 HI30 BV785 AB_2563128 N/D link
LY6G-BV570 LY6G * BioLegend 127629 1A8 BV570 AB_10899738 N/D link

*** Show positive/correct signals in mutliple samples/tissues as judged by eye

** Show positive/correct signals in some but not all samples tested as judged by eye

* show no signal or obviously incorrect signals in all samples tested

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Funded by NIH grants P50-GM107618, U54-HL127365, U2C-CA233262, U2C-CA233280, U54-CA225088, the Ludwig Center at Harvard, and the Ludwig Cancer Research Foundation
©2020, Laboratory of Systems Pharmacology. All rights reserved.
Please cite this resource as, RRID:SCR_016267