Antibodies Tested for Tissue-based CyCIF (Master table, May 2018)
You can navigate through, search, and sort (by clicking the table column heading) the following table as well as download a spreadsheet copy.
This work is made available under a Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) License
Antibodies have been tested for a mimum level of perfomance as descrbed in footnotes below; this should not be assumed to suggest “validation” of the antibody
Search Antibodies
| Antibody Name | Target Protein | Performance | Vendor | Catalog no. | Clone | Fluorophore | Research Resource Identifier | FILTER SET | Note | Link |
|---|---|---|---|---|---|---|---|---|---|---|
| Bax-488 | Bax | *** | BioLegend | 633603 | 2D2 | Alexa Fluor 488 | AB_2562171 | 488/FITC | link | |
| CD11b-488 | CD11b | *** | Abcam | AB204271 | EPR1344 | Alexa Fluor 488 | 488/FITC | link | ||
| CD4-488 | CD4 | *** | R&D Systems | FAB8165G | Polyclonal | Alexa Fluor 488 | 488/FITC | link | ||
| CD8a-488 | CD8 | *** | eBioscience | 53-0008-80 | AMC908 | Alexa Fluor 488 | AB_2574412 | 488/FITC | link | |
| cJUN-488 | cJUN | *** | Abcam | AB193780 | E254 | Alexa Fluor 488 | 488/FITC | link | ||
| CK18-488 | Cytokeratin 18 | *** | eBioscience | 53-9815-80 | LDK18 | Alexa Fluor 488 | AB_2574480 | 488/FITC | link | |
| CK8-FITC | Cytokeratin 8 | *** | eBioscience | 11-9938-80 | LP3K | FITC | AB_10548518 | 488/FITC | link | |
| CycD1-488 | CycD1 | *** | Abcam | AB190194 | EPR2241 | Alexa Fluor 488 | 488/FITC | link | ||
| Ecad-488 | E-Cadherin | *** | CST | 3199 | 24E10 | Alexa Fluor 488 | AB_10691457 | 488/FITC | link | |
| EGFR-488 | EGFR | *** | CST | 5616 | D38B1 | Alexa Fluor 488 | AB_10691853 | 488/FITC | link | |
| EpCAM-488 | EpCAM | *** | CST | 5198 | VU1D9 | Alexa Fluor 488 | AB_10692105 | 488/FITC | link | |
| HES1-488 | HES1 | *** | Abcam | AB196328 | EPR4226 | Alexa Fluor 488 | 488/FITC | link | ||
| Ki67-488 | Ki67 | *** | CST | 11882 | D3B5 | Alexa Fluor 488 | AB_2687824 | 488/FITC | link | |
| LaminA/C-488 | Lamin A/C | *** | CST | 8617 | 4C11 | Alexa Fluor 488 | AB_10997529 | 488/FITC | link | |
| LaminB1-488 | Lamin B1 | *** | Abcam | AB194106 | EPR8985(B) | Alexa Fluor 488 | 488/FITC | link | ||
| mCD3E-FITC | ms_CD3E | *** | BioLegend | 100306 | 145-2C11 | FITC | AB_312671 | 488/FITC | link | |
| mCD4-488 | ms_CD4 | *** | BioLegend | 100532 | RM4-5 | Alexa Fluor 488 | AB_493373 | 488/FITC | link | |
| MET-488 | c-MET | *** | CST | 8494 | D1C2 | Alexa Fluor 488 | AB_10999405 | 488/FITC | link | |
| mF4/80-488 | ms_F4/80 | *** | BioLegend | 123120 | BM8 | Alexa Fluor 488 | AB_893479 | 488/FITC | link | |
| MITF-488 | MITF | *** | Abcam | AB201675 | D5 | Alexa Fluor 488 | 488/FITC | link | ||
| Ncad-488 | N-Cadherin | *** | BioLegend | 350809 | 8C11 | Alexa Fluor 488 | AB_11218797 | 488/FITC | link | |
| p53-488 | p53 | *** | CST | 5429 | 7F5 | Alexa Fluor 488 | AB_10695458 | 488/FITC | link | |
| PCNA-488 | PCNA | *** | CST | 8580 | PC10 | Alexa Fluor 488 | AB_11178664 | 488/FITC | link | |
| PD1-488 | PD1 | *** | CST | 15131 | D3W4U | Alexa Fluor 488 | 488/FITC | Discontinued | link | |
| PDI-488 | PDI | *** | CST | 5051 | C81H6 | Alexa Fluor 488 | AB_10950503 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | *** | CST | 4344 | D13.14.4E | Alexa Fluor 488 | AB_10695876 | 488/FITC | Discontinued | link |
| pNDG1-488 | pNDG1(T346) | *** | CST | 6992 | D98G11 | Alexa Fluor 488 | AB_10827648 | 488/FITC | link | |
| POL2A-488 | POL2A | *** | Novus Biologicals | NB200-598AF488 | 4H8 | Alexa Fluor 488 | AB_2167465 | 488/FITC | link | |
| pS6(S240/244)-488 | pS6(240/244) | *** | CST | 5018 | D68F8 | Alexa Fluor 488 | AB_10695861 | 488/FITC | link | |
| S100a-488 | S100alpha | *** | Abcam | AB207367 | EPR5251 | Alexa Fluor 488 | 488/FITC | link | ||
| SQSTM1-488 | SQSTM1/p62 | *** | CST | 8833 | D1D9E3 | Alexa Fluor 488 | 488/FITC | Discontinued | link | |
| STAT3-488 | STAT3 | *** | CST | 14047 | B3Z2G | Alexa Fluor 488 | 488/FITC | link | ||
| Survivin-488 | Survivin | *** | CST | 2810 | 71G4B7 | Alexa Fluor 488 | AB_10691462 | 488/FITC | link | |
| Catenin-488 | β-Catenin | *** | CST | 2849 | L54E2 | Alexa Fluor 488 | AB_10693296 | 488/FITC | link | |
| Actin-555 | Actin | *** | CST | 8046 | 13E5 | Alexa Fluor 555 | AB_11179208 | 555/Cy3 | link | |
| CD11c-570 | CD11c | *** | eBioscience | 41-9761-80 | 118/A5 | eFluor 570 | AB_2573632 | 555/Cy3 | link | |
| CD3D-555 | CD3D | *** | Abcam | AB208514 | EP4426 | Alexa Fluor 555 | 555/Cy3 | link | ||
| CD4-570 | CD4 | *** | eBioscience | 41-2444-80 | N1UG0 | eFluor 570 | AB_2573601 | 555/Cy3 | link | |
| CD45-PE | CD45 | *** | R&D Systems | FAB1430P-100 | 2D1 | PE | AB_2237898 | 555/Cy3 | link | |
| CK7-555 | Cytokeratin 7 | *** | Abcam | AB209601 | EPR17078 | Alexa Fluor 555 | 555/Cy3 | link | ||
| cMYC-555 | cMYC | *** | Abcam | AB201780 | Y69 | Alexa Fluor 555 | 555/Cy3 | link | ||
| E2F1-555 | E2F1 | *** | Abcam | AB208078 | EPR3818(3) | Alexa Fluor 555 | 555/Cy3 | link | ||
| Ecad-555 | E-Cadherin | *** | CST | 4295 | 24E10 | Alexa Fluor 555 | 555/Cy3 | Discontinued | link | |
| EpCAM-PE | EpCAM | *** | BioLegend | 324205 | 9C4 | PE | AB_756079 | 555/Cy3 | link | |
| FOXO1a-555 | FOXO1a | *** | Abcam | AB207244 | EP927Y | Alexa Fluor 555 | 555/Cy3 | link | ||
| FOXP3-570 | FOXP3 | *** | eBioscience | 41-4777-80 | 236A/E7 | eFluor 570 | AB_2573608 | 555/Cy3 | link | |
| GFAP-570 | GFAP | *** | eBioscience | 41-9892-80 | GA5 | eFluor 570 | AB_2573655 | 555/Cy3 | link | |
| HSP90-PE | HSP90b | *** | Abcam | AB115641 | Polyclonal | PE | AB_10936222 | 555/Cy3 | link | |
| KAP1-594 | KAP1 | *** | BioLegend | 619304 | 20A1 | Alexa Fluor 594 | AB_2563298 | 555/Cy3 | Hard to inactivate | link |
| Keratin-555 | pan-Keratin | *** | CST | 3478 | C11 | Alexa Fluor 555 | AB_10829040 | 555/Cy3 | link | |
| Keratin-570 | pan-Keratin | *** | eBioscience | 41-9003-80 | AE1/AE3 | eFluor 570 | AB_11217482 | 555/Cy3 | link | |
| Ki67-570 | Ki67 | *** | eBioscience | 41-5699-80 | 20Raj1 | eFluor 570 | AB_11220088 | 555/Cy3 | link | |
| LC3-555 | LC3 | *** | CST | 13173 | D3U4C | Alexa Fluor 555 | 555/Cy3 | link | ||
| MAP2-570 | MAP2 | *** | eBioscience | 41-9763-80 | AP20 | eFluor 570 | AB_2573634 | 555/Cy3 | link | |
| pAUR-555 | pAUR1/2/3(T288/T232/T198) | *** | CST | 13464 | D13A11 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pCHK2-PE | pChk2(T68) | *** | CST | 12812 | C13C1 | PE | 555/Cy3 | link | ||
| PDL1-555 | PD-L1/CD274 | *** | Abcam | AB213358 | 28-8 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pH3-555 | pH3(S10) | *** | CST | 3475 | D2C8 | Alexa Fluor 555 | AB_10694639 | 555/Cy3 | link | |
| pRB-555 | pRB(S807/811) | *** | CST | 8957 | D20B12 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pS6(235/236)-555 | pS6(235/236) | *** | CST | 3985 | D57.2.2E | Alexa Fluor 555 | AB_10693792 | 555/Cy3 | Discontinued | link |
| pSRC-PE | pSRC(Y418) | *** | eBioscience | 12-9034-41 | SC1T2M3 | PE | AB_2572680 | 555/Cy3 | link | |
| S6-555 | S6 | *** | CST | 6989 | 54D2 | Alexa Fluor 555 | AB_10828226 | 555/Cy3 | link | |
| SQSTM1-555 | SQSTM1/p62 | *** | Abcam | AB203430 | EPR4844 | Alexa Fluor 555 | 555/Cy3 | link | ||
| VEGFR2-555 | VEGFR2 | *** | CST | 12872 | D5B1 | Alexa Fluor 555 | 555/Cy3 | Discontinued | link | |
| VEGFR2-PE | VEGFR2 | *** | CST | 12634 | D5B1 | PE | 555/Cy3 | link | ||
| Vimentin-555 | Vimentin | *** | CST | 9855 | D21H3 | Alexa Fluor 555 | AB_10859896 | 555/Cy3 | link | |
| Vinculin-570 | Vinculin | *** | eBioscience | 41-9777-80 | 7F9 | eFluor 570 | AB_2573646 | 555/Cy3 | link | |
| gH2ax-PE | gH2ax | *** | BioLegend | 613412 | 2F3 | PE | AB_2616871 | 555/Cy5 | link | |
| AKT-647 | AKT | *** | CST | 5186 | C67E7 | Alexa Fluor 647 | AB_10695877 | 647/Cy5 | link | |
| aSMA-660 | aSMA | *** | eBioscience | 50-9760-80 | 1A4 | eFluor 660 | AB_2574361 | 647/Cy5 | link | |
| B220-647 | CD45R/B220 | *** | BioLegend | 103226 | RA3-6B2 | Alexa Fluor 647 | AB_389330 | 647/Cy5 | link | |
| Bcl2-647 | Bcl2 | *** | BioLegend | 658705 | 100 | Alexa Fluor 647 | AB_2563279 | 647/Cy5 | link | |
| Catenin-647 | Beta-Catenin | *** | CST | 4627 | L54E2 | Alexa Fluor 647 | AB_10691326 | 647/Cy5 | link | |
| CD20-660 | CD20 | *** | eBioscience | 50-0202-80 | L26 | eFluor 660 | AB_11151691 | 647/Cy5 | link | |
| CD45-647 | CD45 | *** | BioLegend | 304020 | HI30 | Alexa Fluor 647 | AB_493034 | 647/Cy5 | link | |
| CD8a-660 | CD8 | *** | eBioscience | 50-0008-80 | AMC908 | eFluor 660 | AB_2574148 | 647/Cy5 | link | |
| CK5-647 | Cytokeratin 5 | *** | Abcam | AB193895 | EP1601Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| CoIIV-647 | Collagen IV | *** | eBioscience | 51-9871-80 | 1042 | Alexa Fluor 647 | AB_10854267 | 647/Cy5 | link | |
| COXIV-647 | COXIV | *** | CST | 7561 | 3E11 | Alexa Fluor 647 | AB_10994876 | 647/Cy5 | link | |
| cPARP-647 | cPARP | *** | CST | 6987 | D64E10 | Alexa Fluor 647 | AB_10858215 | 647/Cy5 | link | |
| FOXA2-660 | FOXA2 | *** | eBioscience | 50-4778-82 | 3C10 | eFluor 660 | AB_2574221 | 647/Cy5 | link | |
| FOXP3-647 | FOXP3 | *** | BioLegend | 320113 | 206D | Alexa Fluor 647 | AB_439753 | 647/Cy5 | link | |
| gH2ax-647 | H2ax(S139) | *** | CST | 9720 | 20E3 | Alexa Fluor 647 | AB_10692910 | 647/Cy5 | link | |
| gH2ax-647 | H2ax(S139) | *** | BioLegend | 613407 | 2F3 | Alexa Fluor 647 | AB_2114994 | 647/Cy5 | link | |
| HES1-647 | HES1 | *** | Abcam | AB196577 | EPR4226 | Alexa Fluor 647 | 647/Cy5 | link | ||
| Ki67-647 | Ki67 | *** | CST | 12075 | D3B5 | Alexa Fluor 647 | 647/Cy5 | link | ||
| Ki67-647 | Ki67 | *** | BioLegend | 350509 | Ki-67 | Alexa Fluor 647 | AB_10900810 | 647/Cy5 | link | |
| mCD45-647 | ms_CD45 | *** | BioLegend | 103124 | 30-F11 | Alexa Fluor 647 | AB_493533 | 647/Cy5 | link | |
| mCD4-647 | ms_CD4 | *** | BioLegend | 100426 | GK1.5 | Alexa Fluor 647 | AB_493519 | 647/Cy5 | link | |
| mEPCAM-647 | ms_EPCAM | *** | BioLegend | 118211 | G8.8 | Alexa Fluor 647 | AB_1134104 | 647/Cy5 | link | |
| MHCI-647 | MHCI/HLAA | *** | Abcam | AB199837 | EP1395Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| MHCII-647 | MHCII | *** | Abcam | AB201347 | EPR11226 | Alexa Fluor 647 | 647/Cy5 | link | ||
| mLy6C-647 | ms_Ly6C | *** | BioLegend | 128009 | HK1.4 | Alexa Fluor 647 | AB_1236551 | 647/Cy5 | link | |
| mTOR-647 | mTOR | *** | CST | 5048 | 7C10 | Alexa Fluor 647 | AB_10828101 | 647/Cy5 | link | |
| NFkB-647 | NFkB (p65) | *** | Abcam | AB190589 | E379 | Alexa Fluor 647 | 647/Cy5 | link | ||
| NGFR-647 | NGFR/CD271 | *** | Abcam | AB195180 | EP1039Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| NUP98-647 | NUP98 | *** | CST | 13393 | C39A3 | Alexa Fluor 647 | 647/Cy5 | link | ||
| p21-647 | p21 | *** | CST | 8587 | 12D1 | Alexa Fluor 647 | AB_10892861 | 647/Cy5 | link | |
| p27-647 | p27 | *** | Abcam | AB194234 | Y236 | Alexa Fluor 647 | 647/Cy5 | link | ||
| pATM-660 | pATM(S1981) | *** | eBioscience | 50-9046-41 | 10H11.E12 | eFluor 660 | AB_2574312 | 647/Cy5 | link | |
| PAX8-647 | PAX8 | *** | Abcam | AB215953 | EPR18715 | Alexa Fluor 647 | 647/Cy5 | link | ||
| PDL1-647 | PD-L1/CD274 | *** | CST | 15005 | E1L3N | Alexa Fluor 647 | 647/Cy5 | link | ||
| pMK2-647 | pMK2(T334) | *** | CST | 4320 | 27B7 | Alexa Fluor 647 | AB_10695401 | 647/Cy5 | Discontinued | link |
| pmTOR-660 | pmTOR(S2448) | *** | eBioscience | 50-9718-41 | MRRBY | eFluor 660 | AB_2574351 | 647/Cy5 | link | |
| pS6_235-647 | pS6(S235/S236) | *** | CST | 4851 | D57.2.2E | Alexa Fluor 647 | AB_10695457 | 647/Cy5 | link | |
| pSTAT3-647 | pSTAT3(Y705) | *** | CST | 4324 | D3A7 | Alexa Fluor 647 | AB_10694637 | 647/Cy5 | link | |
| pTyr-647 | p-Tyrosine | *** | CST | 9415 | p-Tyr-100 | Alexa Fluor 647 | AB_10693160 | 647/Cy5 | link | |
| S100A4-647 | S100A4 | *** | Abcam | AB196168 | EPR2761(2) | Alexa Fluor 647 | 647/Cy5 | link | ||
| Survivin-647 | Survivin | *** | CST | 2866 | 71G4B7 | Alexa Fluor 647 | AB_10698609 | 647/Cy5 | link | |
| TUBB3-647 | TUBB3 | *** | BioLegend | 657405 | AA10 | Alexa Fluor 647 | AB_2563609 | 647/Cy5 | link | |
| Tubulin-647 | beta-Tubulin | *** | CST | 3624 | 9F3 | Alexa Fluor 647 | AB_10694204 | 647/Cy5 | link | |
| Vimentin-647 | Vimentin | *** | BioLegend | 677807 | O91D3 | Alexa Fluor 647 | AB_2616801 | 647/Cy5 | link | |
| anti-14-3-3 | 14-3-3 | *** | Santa Cruz | SC-629-G | Polyclonal | N/D | AB_630820 | N/D | link | |
| anti-53BP1 | 53BP1 | *** | Bethyl | A303-906A | Polyclonal | N/D | AB_2620256 | N/D | link | |
| anti-5HMC | 5HMC | *** | Active Motif | 39769 | Polyclonal | N/D | AB_10013602 | N/D | link | |
| anti-CD11b | CD11b | *** | Abcam | AB133357 | EPR1344 | N/D | AB_2650514 | N/D | link | |
| anti-CD2 | CD2 | *** | Abcam | AB37212 | Polyclonal | N/D | AB_726228 | N/D | link | |
| anti-CD20 | CD20 | *** | Dako | M0755 | L26 | N/D | AB_2282030 | N/D | link | |
| anti-CD3 | CD3 | *** | Dako | A0452 | Polyclonal | N/D | AB_2335677 | N/D | link | |
| anti-CD4 | CD4 | *** | Dako | M7310 | 4B12 | N/D | N/D | link | ||
| anti-CD45RO | CD45RO | *** | Dako | M0742 | UCHL1 | N/D | AB_2237910 | N/D | link | |
| anti-CD8 | CD8 | *** | Dako | M7103 | C8/144B | N/D | AB_2075537 | N/D | link | |
| anti-CycA2 | CycA2 | *** | Abcam | AB38 | E23.1 | N/D | AB_304084 | N/D | link | |
| anti-ET1 | ET-1 | *** | Abcam | AB2786 | TR.ET.48.5 | N/D | AB_303299 | N/D | link | |
| anti-FAP | FAP | *** | eBioscience | BMS168 | F11-24 | N/D | AB_10597443 | N/D | link | |
| anti-FOXP3 | FOXP3 | *** | BioLegend | 320102 | 206D | N/D | AB_430881 | N/D | link | |
| anti-LAMP2 | LAMP2 | *** | Abcam | AB25631 | H4B4 | N/D | AB_470709 | N/D | link | |
| anti-MCM6 | MCM6 | *** | Santa Cruz | SC-9843 | Polyclonal | N/D | AB_2142543 | N/D | link | |
| anti-PAX8 | PAX8 | *** | Abcam | AB191870 | EPR18715 | N/D | N/D | link | ||
| anti-PD1 | PD1 | *** | CST | 86163 | D4W2J | N/D | N/D | link | ||
| anti-pEGFR | pEGFR(Y1068) | *** | CST | 3777 | D7A5 | N/D | AB_2096270 | N/D | link | |
| anti-pERK | pERK(T202/Y204) | *** | CST | 4370 | D13.14.4E | N/D | AB_2315112 | N/D | link | |
| anti-pRB | pRB(S807/811) | *** | Santa Cruz | SC-16670 | Polyclonal | N/D | AB_655250 | N/D | Discontinued | link |
| anti-pRPA32 | pRPA32 (S4/S8) | *** | Bethyl | IHC-00422 | Polyclonal | N/D | AB_1659840 | N/D | link | |
| anti-pSTAT3 | pSTAT3 | *** | CST | 9145 | D3A7 | N/D | AB_2491009 | N/D | link | |
| anti-pTyr | pTyr | *** | CST | 9411 | p-Tyr-100 | N/D | AB_331228 | N/D | link | |
| anti-RPA32 | RPA32 | *** | Bethyl | IHC-00417 | Polyclonal | N/D | AB_1659838 | N/D | link | |
| anti-TPCN2 | TPCN2 | *** | NOVUSBIO | NBP1-86923 | Polyclonal | N/D | AB_11021735 | N/D | link | |
| anti-VEGFR1 | VEGFR1/FLT1 | *** | Santa Cruz | SC-31173 | Polyclonal | N/D | AB_2106885 | N/D | link | |
| Abeta-488 | Beta-Amyloid (1-16) | ** | BioLegend | 803013 | 6E10 | Alexa Fluor 488 | AB_2564765 | 488/FITC | link | |
| BRAF-FITC | B-RAF | ** | Abcam | ab175637 | K21-F | FITC | 488/FITC | link | ||
| BrdU-488 | BrdU | ** | BioLegend | 364105 | 3D4 | Alexa Fluor 488 | AB_2564499 | 488/FITC | link | |
| cCasp3-488 | cCasp3 | ** | R&D Systems | IC835G-025 | 269518 | Alexa Fluor 488 | 488/FITC | link | ||
| CD11b-488 | CD11b | ** | BioLegend | 101219 | M1/70 | Alexa Fluor 488 | AB_493545 | 488/FITC | link | |
| CD123-488 | CD123 | ** | BioLegend | 306035 | 6H6 | Alexa Fluor 488 | AB_2629569 | 488/FITC | link | |
| CD49b-FITC | CD49b | ** | BioLegend | 359305 | P1E6-C5 | FITC | AB_2562530 | 488/FITC | link | |
| CD69-FITC | CD69 | ** | BioLegend | 310904 | FN50 | FITC | AB_314839 | 488/FITC | link | |
| CD71-FITC | CD71 | ** | BioLegend | 334103 | CY1G4 | FITC | AB_1236432 | 488/FITC | link | |
| CD80-FITC | CD80 | ** | R&D Systems | FAB140F | 37711 | FITC | AB_357027 | 488/FITC | link | |
| CD8a-488 | CD8a | ** | eBioscience | 53-0086-41 | OKT8 | Alexa Fluor 488 | AB_10547060 | 488/FITC | link | |
| CDC2-FITC | CDC2/p34 | ** | Santa Cruz | SC-54 FITC | 17 | FITC | AB_627224 | 488/FITC | link | |
| CycB1-FITC | CycB1 | ** | Santa Cruz | SC-752 FITC | Polyclonal | FITC | AB_2072134 | 488/FITC | link | |
| FN-488 | Fibronection | ** | Abcam | AB198933 | F1 | Alexa Fluor 488 | 488/FITC | link | ||
| IFNG-488 | Interferron-Gamma | ** | BioLegend | 502517 | 4S.B3 | Alexa Fluor 488 | AB_493030 | 488/FITC | link | |
| IL1-FITC | IL1 | ** | BioLegend | 511705 | H1b-98 | FITC | AB_1236434 | 488/FITC | link | |
| IL6-FITC | IL6 | ** | BioLegend | 501103 | MQ2-13A5 | FITC | AB_315151 | 488/FITC | link | |
| mCD31-FITC | ms_CD31 | ** | eBioscience | 11-0311-82 | 390 | FITC | AB_465012 | 488/FITC | link | |
| mCD8a-488 | ms_CD8a | ** | BioLegend | 100726 | 53-6.7 | Alexa Fluor 488 | AB_493423 | 488/FITC | link | |
| Nestin-488 | Nestin | ** | eBioscience | 53-9843-80 | 10C2 | Alexa Fluor 488 | AB_1834347 | 488/FITC | link | |
| NeuN-488 | NeuN | ** | Millipore | MAB377X | A60 | Alexa Fluor 488 | AB_2149209 | 488/FITC | link | |
| PR-488 | PR/PGR | ** | Abcam | AB199224 | YR85 | Alexa Fluor 488 | 488/FITC | link | ||
| Snail1-488 | Snail1 | ** | eBioscience | 53-9859-80 | 20C8 | Alexa Fluor 488 | AB_2574482 | 488/FITC | link | |
| TGFB-FITC | TGFB1 | ** | BioLegend | 349605 | TW4-2F8 | FITC | AB_10679043 | 488/FITC | link | |
| TNFa-488 | TNFa | ** | BioLegend | 502917 | MAb11 | Alexa Fluor 488 | AB_493122 | 488/FITC | link | |
| AR-555 | AR | ** | CST | 8956 | D6F11 | Alexa Fluor 555 | AB_11129223 | 555/Cy3 | link | |
| CD11a-PE | CD11a | ** | BioLegend | 301207 | HI111 | PE | AB_314145 | 555/Cy3 | link | |
| CD11b-555 | CD11b | ** | Abcam | AB206616 | EPR1344 | Alexa Fluor 555 | 555/Cy3 | link | ||
| CD131-PE | CD131 | ** | BD | 559920 | JORO50 | PE | AB_397374 | 555/Cy3 | link | |
| CD14-PE | CD14 | ** | eBioscience | 12-0149 | 61D3 | PE | AB_10597598 | 555/Cy3 | link | |
| CD1a-PE | CD1a | ** | BioLegend | 300105 | HI149 | PE | AB_314019 | 555/Cy3 | link | |
| CD1c-PE | CD1c | ** | BioLegend | 331505 | L161 | PE | AB_1089000 | 555/Cy3 | link | |
| CD20-PE | CD20 | ** | BioLegend | 302305 | 2H7 | PE | AB_314253 | 555/Cy3 | link | |
| CD23-PE | CD23 | ** | eBioscience | 12-0232-81 | B3B4 | PE | AB_465592 | 555/Cy3 | link | |
| CD31-PE | CD31 | ** | eBioscience | 12-0319-41 | WM-59 | PE | AB_10670623 | 555/Cy3 | link | |
| CD31-PE | CD31 | ** | R&D Systems | FAB3567P-025 | 9G11 | PE | AB_2279388 | 555/Cy3 | link | |
| CD34-PE | CD34 | ** | Abcam | AB30377 | QBEND/10 | PE | AB_726407 | 555/Cy3 | link | |
| CD45R-e570 | CD45R/B220 | ** | eBioscience | 41-0452-80 | RA3-6B2 | eFluor 570 | AB_2573598 | 555/Cy3 | link | |
| CD71-PE | CD71 | ** | eBioscience | 12-0711-81 | R17217 | PE | AB_465739 | 555/Cy3 | link | |
| CD86-PE | CD86 | ** | BioLegend | 305405 | IT2.2 | PE | AB_314525 | 555/Cy3 | link | |
| CK19-570 | Cytokeratin 19 | ** | eBioscience | 41-9898-80 | BA17 | eFluor 570 | AB_11218678 | 555/Cy3 | link | |
| HER2-570 | HER2 | ** | eBioscience | 41-9757-80 | MJD2 | eFluor 570 | AB_2573628 | 555/Cy3 | link | |
| IL3-PE | IL3 | ** | BD | 554383 | MP2-8F8 | PE | AB_395358 | 555/Cy3 | link | |
| NFATc1-PE | NFATc1 | ** | BioLegend | 649605 | 7A6 | PE | AB_2562546 | 555/Cy3 | link | |
| PDL1-PE | PD-L1/CD274 | ** | BioLegend | 329705 | 29E.2A3 | PE | AB_940366 | 555/Cy3 | link | |
| pMAPK (T202/Y204) | pERK1/2(T202/Y204) | ** | CST | 14095 | 197G2 | PE | 555/Cy3 | link | ||
| pMAPK (Y204/Y187) | pERK1/2(Y204/Y187) | ** | CST | 75165 | D1H6G | PE | 555/Cy3 | link | ||
| pSTAT1-PE | pSTAT1(Y705) | ** | BioLegend | 686403 | A15158B | PE | AB_2616938 | 555/Cy3 | link | |
| ABCC1-647 | ABCC1 | ** | BioLegend | 370203 | QCRL-2 | Alexa Fluor 647 | AB_2566664 | 647/Cy5 | link | |
| AnnexinV-674 | N/D | ** | BioLegend | 640911 | NA | Alexa Fluor 647 | AB_2561293 | 647/Cy5 | link | |
| CD103-647 | CD103 | ** | BioLegend | 350209 | Ber-ACT8 | Alexa Fluor 647 | AB_10640870 | 647/Cy5 | link | |
| CD25-647 | CD25 | ** | BioLegend | 302617 | BC96 | Alexa Fluor 647 | AB_493046 | 647/Cy5 | link | |
| CD31-APC | CD31 | ** | eBioscience | 17-0319-41 | WM-59 | APC | AB_10853188 | 647/Cy5 | link | |
| CD68-APC | CD68 | ** | BioLegend | 333809 | Y1/82A | APC | AB_10567107 | 647/Cy5 | link | |
| CD8a-647 | CD8a | ** | BioLegend | 344725 | SK1 | Alexa Fluor 647 | AB_2563451 | 647/Cy5 | link | |
| CD8a-647 | CD8a | ** | R&D Systems | FAB1509R-025 | 37006 | Alexa Fluor 647 | 647/Cy5 | link | ||
| CycE-660 | CycE | ** | eBioscience | 50-9714-80 | HE12 | eFluor 660 | AB_2574350 | 647/Cy5 | link | |
| HIF1-647 | HIF1 | ** | BioLegend | 359705 | 546-16 | Alexa Fluor 647 | AB_2563331 | 647/Cy5 | link | |
| HP1-647 | HP1 | ** | Abcam | AB198391 | EPR5777 | Alexa Fluor 647 | 647/Cy5 | link | ||
| mCD123-APC | ms_CD123 | ** | eBioscience | 17-1231-81 | 5B11 | APC | AB_891363 | 647/Cy5 | link | |
| NGFR-647 | NGFR/CD271 | ** | BD | 560326 | C40-1457 | Alexa Fluor 647 | AB_1645403 | 647/Cy5 | link | |
| pBTK-660 | pBTK(Y551/Y511) | ** | eBioscience | 50-9015-80 | M4G3LN | eFluor 660 | AB_2574306 | 647/Cy5 | link | |
| PD1-647 | PD1 | ** | Abcam | AB201825 | EPR4877 (2) | Alexa Fluor 647 | 647/Cy5 | link | ||
| PR-660 | PR/PGR | ** | eBioscience | 50-9764-80 | KMC912 | eFluor 660 | AB_2574363 | 647/Cy5 | link | |
| RUNX3-660 | RUNX3 | ** | eBioscience | 50-9817-80 | R3-5G4 | eFluor 660 | AB_2574383 | 647/Cy5 | link | |
| SOX2-647 | SOX2 | ** | Abcam | AB192075 | Polyclonal | Alexa Fluor 647 | 647/Cy5 | link | ||
| anti-53BP1 | 53BP1 | ** | Millipore | MAB3802 | BP13 | N/D | AB_2206767 | N/D | link | |
| anti-Axl | Axl | ** | R&D | AF154 | Polyclonal | N/D | AB_354852 | N/D | link | |
| anti-CD11b | CD11b | ** | Abcam | AB52478 | EP1345Y | N/D | AB_868788 | N/D | link | |
| anti-CD8a | CD8 | ** | eBioscience | 14-0085-80 | C8/144B | N/D | AB_11151339 | N/D | link | |
| anti-CEP170 | CEP170 | ** | Abcam | AB72505 | Polyclonal | N/D | AB_1268101 | N/D | link | |
| anti-cMYC | cMYC | ** | BioLegend | 626801 | 9E10 | N/D | AB_2235686 | N/D | link | |
| anti-CPS1 | CPS1 | ** | Abcam | AB129076 | EPR7493-3 | N/D | AB_11156290 | N/D | link | |
| anti-E2F1 | E2F1 | ** | ThermoFisher | MS-879-P1 | KH95 | N/D | AB_143934 | N/D | link | |
| anti-eEF2K | eEF2K | ** | Santa Cruz | SC-21642 | K-19 | N/D | AB_640043 | N/D | link | |
| anti-Emil1 | Emil1 | ** | Abcam | AB212397 | EMIL/1176 | N/D | N/D | link | ||
| anti-FKHRL1 | FKHRL1 | ** | Santa Cruz | SC-9812 | Polyclonal | N/D | AB_640608 | N/D | Discontinued | link |
| anti-FLAG | FLAG | ** | Sigma | F1804 | M2 | N/D | AB_262044 | N/D | link | |
| anti-GranB | Granzyme_B | ** | Dako | M7235 | M7235 | N/D | AB_2114697 | N/D | link | |
| anti-HMB45 | HMB45 | ** | Abcam | AB732 | HMB45+M2-7C10+M2-9E3 | N/D | AB_305844 | N/D | link | |
| anti-HSP90b | HSP90b | ** | Santa Cruz | SC-1057 | D-19 | N/D | AB_2121392 | N/D | link | |
| anti-IL2Ra | IL2Ra | ** | Abcam | AB128955 | EPR6452 | N/D | AB_11141054 | N/D | link | |
| anti-LAMP2 | LAMP2 | ** | R&D | AF6228 | Polyclonal | N/D | AB_10971818 | N/D | link | |
| anti-MITF | MITF | ** | Abcam | AB12039 | C5 | N/D | AB_298801 | N/D | link | |
| anti-Ncad | N-Cadherin | ** | Abcam | AB18203 | Polyclonal | N/D | AB_444317 | N/D | link | |
| anti-NCAM | NCAM | ** | Abcam | AB6123 | ERIC-1 | N/D | AB_2149537 | N/D | link | |
| anti-NF1 | NF1 | ** | Abcam | AB178323 | McNFn27b | N/D | N/D | link | ||
| anti-pCTD | Pol II CTD(S2) | ** | Active Motif | 61083 | 3E10 | N/D | AB_2687450 | N/D | link | |
| anti-PD1 | PD1 | ** | CST | 43248 | EH33 | N/D | N/D | link | ||
| anti-pTuberin | pTuberin(S664) | ** | Abcam | AB133465 | EPR8202 | N/D | AB_11157389 | N/D | link | |
| anti-S100 | S100 | ** | Dako | Z0311 | Polyclonal | N/D | AB_10013383 | N/D | link | |
| anti-SIRT3 | SIRT3 | ** | CST | 2627 | C73E3 | N/D | AB_2188622 | N/D | link | |
| anti-TIA1 | TIA1 | ** | Santa Cruz | SC-1751 | Polyclonal | N/D | AB_2201433 | N/D | link | |
| anti-TLR3 | TLR3 | ** | Santa Cruz | SC-8691 | Polyclonal | N/D | AB_2240700 | N/D | link | |
| anti-TNFa | TNFa | ** | Abcam | AB11564 | MP6-XT3 | N/D | AB_298170 | N/D | link | |
| anti-TPCN2 | TPCN2 | ** | Abcam | AB119915 | Polyclonal | N/D | AB_10903692 | N/D | link | |
| CD11a-FITC | CD11a | * | eBioscience | 11-0119-41 | HI111 | FITC | AB_10597888 | 488/FITC | link | |
| CD20-FITC | CD20 | * | BioLegend | 302303 | 2H7 | FITC | AB_314251 | 488/FITC | link | |
| CD2-FITC | CD2 | * | BioLegend | 300206 | RPA-2.10 | FITC | AB_314030 | 488/FITC | link | |
| CD45RO-488 | CD45RO | * | BioLegend | 304212 | UCHL1 | Alexa Fluor 488 | AB_528823 | 488/FITC | link | |
| CD8a-488 | CD8 | * | BioLegend | 301024 | RPA-T8 | Alexa Fluor 488 | AB_2561282 | 488/FITC | link | |
| cJUN-FITC | cJUN | * | Santa Cruz | SC-1694 FITC | Polyclonal | FITC | AB_631263 | 488/FITC | Discontinued | link |
| CXCR5-FITC | CXCR5 | * | BioLegend | 356913 | J252D4 | FITC | AB_2561895 | 488/FITC | link | |
| Ecad-FITC | Ecad | * | BioLegend | 324103 | 67A4 | FITC | AB_756065 | 488/FITC | link | |
| FOXP3-488 | FOXP3 | * | BioLegend | 320011 | 150D | Alexa Fluor 488 | AB_439747 | 488/FITC | link | |
| MITF-488 | MITF | * | Novus Biologicals | NB100-56561AF488 | 21D1418 | Alexa Fluor 488 | AB_838580 | 488/FITC | link | |
| NCAM-488 | NCAM/CD56 | * | Abcam | AB200333 | EPR2566 | Alexa Fluor 488 | 488/FITC | link | ||
| NCAM-FITC | NCAM/CD56 | * | ThermoFisher | 11-0566-41 | TULY56 | FITC | AB_2572458 | 488/FITC | link | |
| NGFR-FITC | NGFR/CD271 | * | BioLegend | 345103 | ME20.4 | FITC | AB_1937226 | 488/FITC | link | |
| PD1-488 | PD-1 | * | BioLegend | 367407 | NAT105 | Alexa Fluor 488 | AB_2566677 | 488/FITC | link | |
| PD1-488 | PD-1 | * | BioLegend | 329935 | EH12.2H7 | Alexa Fluor 488 | AB_2563593 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | * | CST | 4374 | E10 | Alexa Fluor 488 | AB_10705598 | 488/FITC | link | |
| pERK-488 | pERK(T202/Y204) | * | CST | 4780 | 137F5 | Alexa Fluor 488 | AB_10705598 | 488/FITC | link | |
| S100A4-FITC | S100A4 | * | BioLegend | 370007 | NJ-4F3-D1 | FITC | AB_2572073 | 488/FITC | link | |
| SOX2-488 | SOX2 | * | BioLegend | 656109 | 14A6A34 | Alexa Fluor 488 | AB_2563956 | 488/FITC | link | |
| CD133-PE | CD133 | * | eBioscience | 12-1338-41 | TMP4 | PE | AB_1582258 | 555/Cy3 | link | |
| cMyc-TRITC | cMYC | * | Santa Cruz | SC-40 TRITC | 9E10 | TRITC | AB_627268 | 555/Cy3 | link | |
| cPARP-555 | cPARP | * | CST | 6894 | D64E10 | Alexa Fluor 555 | AB_10830735 | 555/Cy3 | link | |
| CTLA4-PE | CTLA4 | * | BioLegend | 369603 | BNI3 | PE | AB_2566796 | 555/Cy3 | link | |
| GATA3-594 | GATA3 | * | BioLegend | 653816 | 16E10A23 | Alexa Fluor 594 | AB_2563353 | 555/Cy3 | link | |
| GFAP-Cy3 | GFAP | * | Millipore | MAB3402C3 | NA | Cy3 | AB_11213580 | 555/Cy3 | link | |
| Oct4-555 | OCT_4 | * | CST | 4439 | C30A3 | Alexa Fluor 555 | AB_10922586 | 555/Cy3 | link | |
| p21-555 | p21 | * | CST | 8493 | 12D1 | Alexa Fluor 555 | AB_10860074 | 555/Cy3 | link | |
| PD1-PE | PD1 | * | BioLegend | 329905 | EH12.2H7 | PE | AB_940481 | 555/Cy3 | link | |
| PDGFRb-555 | PDGFRb | * | Abcam | AB206874 | Y92 | Alexa Fluor 555 | 555/Cy3 | link | ||
| pSTAT1-555 | pSTAT1 | * | CST | 8183 | 58D6 | Alexa Fluor 555 | AB_10860600 | 555/Cy3 | link | |
| TIM1-PE | TIM1 | * | BioLegend | 353903 | 1D12 | PE | AB_11125165 | 555/Cy3 | link | |
| cCasp3-647 | cCasp3 | * | CST | 9602 | D3E9 | Alexa Fluor 647 | AB_2687881 | 647/Cy5 | link | |
| CD103-APC | CD103 | * | eBioscience | 17-1038-41 | B-Ly7 | APC | AB_10669816 | 647/Cy5 | link | |
| CD3-647 | CD3 | * | BioLegend | 300422 | UCHT1 | Alexa Fluor 647 | AB_493092 | 647/Cy5 | link | |
| CD3-660 | CD3 | * | eBioscience | 50-0037-41 | OKT3 | eFluor 660 | AB_2574150 | 647/Cy5 | link | |
| CD3-APC | CD3 | * | eBioscience | 17-0038-41 | UCHT1 | APC | AB_10804761 | 647/Cy5 | link | |
| CD45RO-APC | CD45RO | * | BioLegend | 304210 | UCHL1 | APC | AB_314426 | 647/Cy5 | link | |
| ER-647 | ER | * | Abcam | AB205851 | EPR4097 | Alexa Fluor 647 | 647/Cy5 | link | ||
| FOXO3a-647 | FOXO3a | * | Abcam | AB196539 | EP1949Y | Alexa Fluor 647 | 647/Cy5 | link | ||
| GZMA-e660 | granzyme A | * | ThermoFisher | 50-9177-41 | CB9 | eFluor 660 | AB_2574330 | 647/Cy5 | link | |
| GZMB-647 | Granzyme_B | * | BioLegend | 515405 | GB11 | Alexa Fluor 647 | AB_2294995 | 647/Cy5 | link | |
| GZMB-APC | Granzyme_B | * | R&D Systems | IC29051A | 356412 | APC | AB_894691 | 647/Cy5 | Discontinued | link |
| HER2-647 | HER2 | * | BioLegend | 324412 | 24D2 | Alexa Fluor 647 | AB_2262300 | 647/Cy5 | link | |
| mCD49b-647 | ms_CD49b | * | BioLegend | 103511 | HMα2 | Alexa Fluor 647 | AB_528830 | 647/Cy5 | link | |
| NCAM-647 | NCAM/CD56 | * | BioLegend | 362513 | 5.1H11 | Alexa Fluor 647 | AB_2564086 | 647/Cy5 | link | |
| NCAM-e660 | NCAM/CD56 | * | ThermoFisher | 50-0565-80 | 5tukon56 | eFluor 660 | AB_2574160 | 647/Cy5 | link | |
| pAKT-647 | pAKT | * | CST | 4075 | D9E | Alexa Fluor 647 | AB_10691856 | 647/Cy5 | link | |
| pERK-647 | pERK (T202/Y204) | * | CST | 4375 | E10 | Alexa Fluor 647 | AB_10706777 | 647/Cy5 | link | |
| pERK-647 | pERK (T202/Y204) | * | BioLegend | 369503 | 6B8B69 | Alexa Fluor 647 | AB_2571895 | 647/Cy5 | link | |
| pIKBa-660 | pIKBa | * | eBioscience | 50-9035-41 | RILYB3R | eFluor 660 | AB_2574310 | 647/Cy5 | link | |
| YAP-647 | YAP | * | CST | 38707S | D8H1X | Alexa Fluor 647 | 647/Cy5 | link | ||
| anit-FANCD2 | FANCD2 | * | Bethyl | IHC-00624 | Polyclonal | N/D | AB_10752755 | N/D | link | |
| anit-pcJUN | p-cJUN | * | Santa Cruz | SC-822 | KM-1 | N/D | AB_627262 | N/D | link | |
| anti-AXL | AXL | * | CST | 8661 | C89E7 | N/D | AB_11217435 | N/D | link | |
| anti-CXCR5 | CXCR5 | * | GeneTex | GTX100351 | Polyclonal | N/D | AB_1240668 | N/D | link | |
| anti-CXCR5 | CXCR5 | * | R&D | MAB-190-SP | 51505 | N/D | AB_2292654 | N/D | link | |
| anti-FOXO3a | FOXO3a | * | CST | 2497 | 75D8 | N/D | AB_836876 | N/D | link | |
| anti-GZMB | Granzyme B | * | Abcam | AB4059 | Polyclonal | N/D | AB_304251 | N/D | link | |
| anti-PD1 | PD-1 | * | Abcam | AB63477 | Polyclonal | N/D | AB_2159165 | N/D | link | |
| anti-PD1 | PD-1 | * | ThermoFisher | 14-9985-81 | J43 | N/D | AB_468663 | N/D | link | |
| anti-PD1 | PD-1 | * | R&D | AF1021 | Polyclonal | N/D | AB_354541 | N/D | link | |
| anti-RFP | RFP | * | ThermoFisher | R10367 | Polyclonal | N/D | AB_2315269 | N/D | link | |
| CD11C-BV570 | CD11C | * | BioLegend | 117331 | N418 | BV570 | AB_10900261 | N/D | link | |
| CD45-BV785 | CD45 | * | BioLegend | 304047 | HI30 | BV785 | AB_2563128 | N/D | link | |
| LY6G-BV570 | LY6G | * | BioLegend | 127629 | 1A8 | BV570 | AB_10899738 | N/D | link |
*** Show positive/correct signals in mutliple samples/tissues as judged by eye
** Show positive/correct signals in some but not all samples tested as judged by eye
* show no signal or obviously incorrect signals in all samples tested