Visualizing and sharing multiplexed CyCIF data

For a ~10 x 11mm tissue FFPE specimen under 20X objective, each cycle of t-CyCIF generates around 160 individual image tiles. The assembly process involves stitching sequential image tiles from a single t-CyCIF cycle into one large image panel, flat-fielding to correct for uneven illumination and registration of images from successive t-CyCIF cycles to each other; these procedures were performed using ImageJ, ASHLAR, and BaSiC software.

These sharable, guided analyses are created by users of all levels of coding experience using the Minerva suite. This includes Minerva Author, a tool that enables the user to easily create a guided analysis and Minerva Story, a tool that enables users to turn their story into a narrative, web-based image for viewing and sharing.

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Melanoma Pre-Cancer Atlas (HTAN)

Unpublished t-CyCIF images of melanoma and precursor fields from two patients. The specimen from patient 1 illustrates different regions of melanoma progression from relatively normal melanocytes to precursor melanocytic dysplasia to invasive melanoma. In addition, the specimen shows different regions representing immune responses to early melanoma (inflammatory regression) as well as to invasive melanoma in the form of a brisk immune infiltrative immune response (tumor infiltrating lymphocytes “TILs”). The excision specimen from patient 2 illustrates the histologic evolution of melanoma from a precursor field to melanoma in situ, and ultimately to polypoidal invasive melanoma.

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Tour of the melanoma dataset

The unpublished 13-plex t-CyCIF images of tissue specimens from four patients with BRAF-mutant metastatic melanoma resected before (left) and after (right) treatment with BRAF and MEK inhibitors (dabrafenib/trametinib). Each image is composed of 150-200 image tiles at a nominal resolution of ~0.9 μm.

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Figures in the 2018 eLife Publication

Tissue-based cyclic immunofluorescent microscopy (t-CyCIF) is a simple method for generating highly multiplexed optical images from formalin-fixed paraffin-embedded (FFPE) tissue samples routinely used for histopathological diagnosis of human disease. The method is based on previously described single-cell imaging approaches and readily implemented on existing instruments (Gerdes et al. 2013, Lin et al. 2015, 2016).

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Keenan et al., 2020 (in preparation)

Selective inhibition of the negative cell cycle regulator WEE1 may enhance the efficacy of DNA-damaging agents by reducing DNA damage repair. These are multiplexed cyclic immunofluorescence on paired pre- and post-WEE1 inhibitor tumor biopsies, from the first phase II study assessing the efficacy of the WEE1 inhibitor adavosertib with cisplatin in metastatic triple-negative breast cancer (mTNBC). Among patients with mTNBC treated with 0-1 prior lines, adavosertib combined with cisplatin narrowly missed the prespecified ORR cutoff of > 30%. The finding of immune infiltrated tumors in patients with clinical benefit to therapy requires validation in future studies.

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Spatio-temporal effects of genetic and therapeutic perturbation on the immune micro-environment in murine models of lung and pancreas adenocarcinomas

In a collaboration with Dr. Tyler Jacks at Ludwig MIT, we used t-CyCIF to characterize tumor intrinsic and extrinsic states in genetically engineered cancer models of lung and pancreas samples treated with clinical trial agents such as HSP90 inhibitors, classic cytotoxic agents, and immune checkpoint blockade modifiers. We are interested in understanding the effects of genetic perturbations, for example targeted deletion or over-expression of individual or panels of cancer, and immune-related genes in these samples.