Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes

Lin JR, Izar B, Wang S, Yapp C, Mei S, Shah P, Santagata S, Sorger PK.

eLife. 2018 Jul 11;7:e31657. PMID: 29993362

Publication summary

Available images

Figure 2

Representative t-CyCIF staining of a large metastatic melanoma lesion and adjacent benign tissue stitched together using the Ashlar software from 165 successive CyteFinder fields using a 20X/0.8NA objective.

Melanoma mosaic
Figure 5 and 6

Representative images of Slide A (top panels) and Slide B specimens (bottom panels) after each t-CyCIF cycle. The color coding highlighting specific cycles is the same as in A.

Tonsil A mossaic Tonsil B mossaic
Figure 7 and 8

t-CyCIF of a large resection specimen from a patient with pancreatic cancer. The entire sample comprising 143 stitched 10X fields of view is shown.

PDAC mosaic
Figure 10

Eight-cycle t-CyCIF of a tissue microarray (TMA) including 13 normal tissues and corresponding tumor types. The TMA includes normal tissue types, and corresponding high and low grade tumors, for a total of 39 specimens.

TMA mosaic
Figure 11 and 12

Molecular heterogeneity in a single GBM tumor. (A) Representative low magnification image of a GBM specimen generated from 221 stitched 10X frames; the sample was subjected to 10 rounds of t-CyCIF.

GBM mosaic

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Funded by NIH grants P50-GM107618, U54-HL127365, U2C-CA233262, U2C-CA233280, U54-CA225088, the Ludwig Center at Harvard, and the Ludwig Cancer Research Foundation
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Please cite this resource as CyCIF.org, RRID:SCR_016267