Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging

Du Z, Lin JR, Rashid R, Maliga Z, Wang S, Aster J, Izar B, Sorger PK, Santagata S

Available images

tonsil_1.jpg
Tonsil 1

Representative t-CyCIF image acquired from a formalin-fixed, paraffin-embedded (FFPE) human tonsil tissue section stitched together using ASHLAR software from 224 fields acquired using a 40X/0.6NA objective.

CyCIF tonsil 1
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IHC Tonsil Sections

IHC tonsil sections
lung_3.jpg
Squamous cell carcinoma of the lung

Representative t-CyCIF image of a squamous cell carcinoma of the lung stitched together using ASHLAR software from 132 fields using a 40X/0.6NA objective.

CyCIF image
lung_1.jpg
Lung adenocarcinoma metastasis to a lymph node

Representative t-CyCIF image of a lung adenocarcinoma metastasis to a lymph node stitched together using ASHLAR software from 80 fields using a 40X/0.6NA objective.

CyCIF image
lung_2.jpg
Lung squamous cell carcinoma metastasis to the brain

Representative t-CyCIF image of a lung squamous cell carcinoma metastasis to the brain stitched together using ASHLAR software from 187 fields using a 40X/0.6NA objective.

CyCIF image