Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging

Du Z, Lin JR, Rashid R, Maliga Z, Wang S, Aster J, Izar B, Sorger PK, Santagata S

Available images

tonsil_1.jpg
TONSIL

Representative t-CyCIF image acquired from a formalin-fixed, paraffin-embedded (FFPE) human tonsil tissue section stitched together using ASHLAR software from 224 fields acquired using a 40X/0.6NA objective.

CyCIF tonsil image
tonsil_ihc.jpg
IHC Tonsil Sections

IHC tonsil sections
lung_3.jpg
LUNG-3-PR: Primary lung squamous cell carcinoma

Representative t-CyCIF image of a squamous cell carcinoma of the lung stitched together using ASHLAR software from 132 fields using a 40X/0.6NA objective.

CyCIF lung-3 image
lung_1.jpg
LUNG-1-LN: Lung adenocarcinoma metastasis to lymph node

Representative t-CyCIF image of a lung adenocarcinoma metastasis to a lymph node stitched together using ASHLAR software from 80 fields using a 40X/0.6NA objective.

CyCIF lung-1 image
lung_2.jpg
LUNG-2-BR: Lung squamous cell carcinoma metastasis to brain

Representative t-CyCIF image of a lung squamous cell carcinoma metastasis to the brain stitched together using ASHLAR software from 187 fields using a 40X/0.6NA objective.

CyCIF lung-2 image