Training

NCI-funded Cancer System Biology Centers (CSBC centers) at Harvard Medical School and Oregon Health & Science University are collaborating to make it possible for others to perform t-CyCIF in their own labs. This includes offering resources through the CyCIF.org website and hands-on training sessions. We expect that several of these sessions will be held per year.

2018 Multiscale Imaging at the Edge: Hands-On Workshops

On May 7-11, 2018 The M2CH Center at Oregon Health & Science University held a hands on training session on correlative light & EM imaging, super resolution microscopy and cyclic immunofluorescence (CycIF). The curriculum included:

• Review CycIF Protocol and Applications
• Tissue preparation (Sample tissue: normal breast)
• Staining - Washing – Quenching Workflows
• AxioImager Image Acquisition
• Image Processing and Analysis
• Zen and Matlab Image Registration, Overlay and Data Visualization