Antibodies for tissue-based CyCIF
To date, we have tested commercial antibodies against ~200 proteins for their compatibility with t-CyCIF. These antibodies include lineage makers, cytoskeletal proteins, cell cycle regulators, the phosphorylated forms of signaling proteins and kinases, transcription factors, markers of cell state including quiescence, senescence, apoptosis, stress, etc.
The qualified list of 300 antibodies used in our foundational tissue CyCIF paper (Lin, et al. eLife 2018).
Representative images of antibody staining patterns for a subset of 41 (and growing) antibodies (Lin, et al. eLife 2018).